Mononucleosis is a viral disease that affects certain blood cells and creates flu-like symptoms. Mononucleosis is a viral infection caused by the Epstein-Bar… At best, the Monospot test may indicate that a person has a typical case of infectious mononucleosis, but does not confirm the presence of EBV infection. Your doctor may order this test if you have symptoms of mononucleosis. A mononucleosis spot (or Monospot) test is a blood test used to determine whether or not you are infected with the Epstein-Barr virus, which is the organism that causes infectious mononucleosis. Interpretation of EBV Antibody … These antibodies show up during or after during... EBV antibody test. This test looks for EBV antibodies, the … He or she will look for signs such as swollen lymph nodes, tonsils, liver or spleen, and consider how these signs relate to the symptoms you describe. Your doctor may suspect mononucleosis based on your signs and symptoms, how long they've lasted and a physical examination. Types of mono tests include: Monospot test. This test looks for specific antibodies in the blood.